新井 博之 博士(農学)
Hiroyuki ARAI Ph.D.
Associate Professor,
Department of Biotechnology,
Graduate School of Agricultural and Life Sciences,
The University of Tokyo

[Note that there are some Hiroyuki ARAIs in the University of Tokyo.
Please do not confuse me with the others.]


連絡先：
　　東京大学大学院農学生命科学研究科
　　応用生命工学専攻 応用微生物学研究室
　　（農学部２号館２１４号室）

　　〒113-8657　東京都文京区弥生１−１−１
　　TEL 03-5841-1741
　　FAX 03-5841-5272
　　E-mail: aharai [at] mail.ecc.u-tokyo.ac.jp (replace [at] with @)


経歴：
　　東京大学大学院博士課程修了・博士（農学）
　　日本学術振興会特別研究員（DC）
　　理化学研究所・基礎科学特別研究員
　　理化学研究所・研究員
　　東京大学大学院・助手
　　テキサス大学ヒューストン医学部・客員教員
　　東京大学大学院・助教
　　東京大学大学院・准教授（現職）


所属学会：
　　日本農芸化学会
　　日本生化学会
　　日本生物工学会
　　日本ゲノム微生物学会
　　環境バイオテクノロジー学会
　　バイオインダストリー協会
　　アメリカ微生物学会(ASM)


主な研究テーマ：
　　　　
Pseudomonas aeruginosa PAO1
（緑膿菌)
　酸素と一酸化窒素(NO)への応答と耐性機構 (ANR, DNR, FhpR)
　好気呼吸遺伝子の発現調節
　脱窒遺伝子の発現調節
　ECFシグマ因子の機能解析
　クオラムセンシング、バイオフィルム、ペリクル

Rhodobacter sphaeroides 2.4.1
（光合成細菌、紅色非硫黄細菌）
　エネルギー代謝変換機構
　酸素、一酸化窒素(NO)への応答機構
　マイクロアレイ解析

Roseobacter denitrificans OCh114
（好気性酸素非発生型光合成細菌、紅色非硫黄細菌）
　好気呼吸、光合成、嫌気呼吸(脱窒)のエネルギー代謝変換機構
　脱窒遺伝子の発現調節
　光脱窒
※基盤研究C(H27-29)に採択されました。

Hydrogenovibrio marinus MH-110
（海洋性水素細菌、絶対独立栄養細菌)
　カルビンサイクルの炭酸固定酵素RubisCOの遺伝子発現調節 (CbbR)
　カルボキシソーム、カルボニックアンヒドラーゼによる炭酸ガス濃縮機構

Hydrogenobacter thermophilus TK-6
（好熱性水素細菌、絶対独立栄養細菌）
　ゲノム解析、トランスクリプトーム解析
　好気代謝と嫌気代謝の転換機構
　脱窒遺伝子と酵素の解析
　還元的TCAサイクルによる炭酸固定

Comamonas testosteroni TA441
(土壌細菌)
　実験室進化によるフェノール資化能の獲得機構
　Viable but not culturable (VNC), Active but not culturable (ANC)
　リグニン関連物質の生分解

Acetobacter aceti
（酢酸菌)
　ゲノム解析、トランスクリプトーム解析、
　エネルギー代謝、炭素代謝解析

受賞等：

　2017年 第18回酵素応用シンポジウム研究奨励賞
　「緑膿菌のストレス耐性に関わるcbb3型シトクロムc酸化酵素の機能解析」

　2001年度　日本農芸化学会　農芸化学奨励賞
　「微生物由来脱窒遺伝子群の発現調節に関する研究」

　1999年度　バイオインダストリー協会　発酵と代謝研究奨励金
　「微生物由来脱窒遺伝子群の発現調節に関する研究」


原著論文：
  1. Specific expression and function of the A-type cytochrome c oxidase under starvation conditions in Pseudomonas aeruginosa
    Tatsuya Osamura, Takuro Kawakami, Reiko Kido, Masaharu Ishii, and Hiroyuki Arai
    PLoS ONE, 112(5): e0177957 (2017)
    doi:  10.1371/journal.pone.0177957
    PubMed
  2. Expression of multiple cbb3 cytochrome c oxidase isoforms by combinations of multiple isosubunits in Pseudomonas aeruginosa
    Takehiro Hirai, Tatsuya Osamura, Masaharu Ishii, and Hiroyuki Arai
    Proc. Natl. Acad. Sci. USA, 113 (45), 12815-12819 (2016)
    doi/10.1073/pnas.1613308113
    PubMed

  3. Metabolic features of Acetobacter aceti
    Hiroyuki Arai, Kenta Sakurai, and Masaharu Ishii
    In “Acetic acid Bacteria: Ecology and physiology” Matsushita, K. et al. (eds.) , pp. 225-271. Springer (2016)
    doi: 10.1007/978-4-431-55933-7_12
    Link

  4. Discovery of an intermolecular disulfide bond required for the thermostability of a heterodimeric protein from the thermophile Hydrogenobacter thermophilus
    Keug Tae Kim, Yoko Chiba, Hiroyuki Arai, and Masaharu Ishii
    Biosci. Biotechnol. Biochem., 80 (2), 232-240 (2016)
    doi: 10.1080/09168451.2015.1079476
    PubMed
  5. Enzymatic characterization and in-vivo function of five terminal oxidases in Pseudomonas aeruginosa
    Hiroyuki Arai, Takuro Kawakami, Tatsuya Osamura, Takehiro Hirai, Yoshiaki Sakai, and Masaharu Ishii
    J Bacteriol., 196 (24), 4206-4215 (2014)
    PubMed
    Journal of Bacteriology誌のCommentaryで紹介されました。
    PubMed

  6. Fine-tuned regulation of the dissimilatory nitrite reductase gene by oxygen and nitric oxide in Pseudomonas aeruginosa
    Miho Kuroki, Yasuo Igarashi, Masaharu Ishii, and Hiroyuki Arai
    Environ. Microbiol. Rep., 6 (6), 792-801 (2014)
    PubMed

  7. The crystal structure of siroheme decarboxylase in complex with iron-uroporphyrin III reveals two essential histidine residues
    Kristin Haufschildt, Stefan Schmelz, Theresa M. Kriegler, Alexander Neumann, Judith Streif, Hiroyuki Arai, Dirk W. Heinz, and Gunhild Layer
    J. Mol. Biol., 426 (19), 3272-3286 (2014)
    PubMed

  8. Adaptation of Hydrogenobacter thermophilus toward oxidative stress triggered by high expression of alkyl hydroperoxide reductase
    Yuya Sato, Hiroyuki Arai, Yasuo Igarashi, and Masaharu Ishii
    Biosci. Biotechnol. Biochem., 78 (9) 1619-1622 (2014)
    PubMed

  9. Structural units important for activity of a novel-type phosphoserine phosphatase from Hydrogenobacter thermophilus TK-6 revealed by crystal structure analysis
    Yoko Chiba, Shoichiro Horita, Jun Ohtsuka, Hiroyuki Arai, Koji Nagata, Yasuo Igarashi, Masaru Tanokura, and Masaharu Ishii
    J. Biol. Chem., 288 (16), 11448-11458 (2013)
    PubMed

  10. Role of nitric oxide-detoxifying enzymes in virulence of Pseudomonas aeruginosa against the silkworm, Bombyx mori
    Hiroyuki Arai and Kazuhiro Iiyama
    Biosci. Biotechnol. Biochem., 77 (1), 198-200 (2013)
    PubMed

  11. Transcriptome response to nitrosative stress in Rhodobacter sphaeroides 2.4.1
    Hiroyuki Arai, Jung Hyeob Roh, Jesus M. Eraso, and Samuel Kaplan
    Biosci. Biotechnol. Biochem., 77 (1), 111-118 (2013)
    PubMed

  12. Role of the glyoxylate pathway in acetic acid production by Acetobacter aceti
    Kenta Sakurai, Shoko Yamazaki, Masaharu Ishii, Yasuo Igarashi, and Hiroyuki Arai
    J. Biosci. Bioeng., 115 (1), 32-36 (2013)
    PubMed

  13. Identification of the genes encoding nitric oxide reductase in the aerobic photosynthetic bacterium Roseobacter denitrificans OCh114
    Makoto Kimura, Masaharu Ishii, Yasuo Igarashi, and Hiroyuki Arai
    Biosci. Biotechnol. Biochem., 76 (10), 1984-1986 (2012)
    PubMed

  14. Transcriptome analyses for metabolic enzymes in thiosulfate- and hydrogen-grown Hydrogenobacter thermophilus cells
    Yuya Sato, Haruna Kanbe, Hirosuke Miyano, Yoshihiro Sambongi, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Biosci. Biotechnol. Biochem., 76 (9), 1677-1681 (2012)
    PubMed

  15. Involvement of flagella-driven motility and pili in Pseudomonas aeruginosa colonization at the air-liquid interface
    Kyosuke Yamamoto, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Microbes Environ., 27 (3), 320-323 (2012)
    PubMed

  16. Crystallization and preliminary X-ray diffraction analysis of a novel type of phosphoserine phosphatase from Hydrogenobacter thermophilus TK-6
    Yoko Chiba, Shoichiro Horita, Jun Ohtsuka, Hiroyuki Arai, Koji Nagata, Yasuo Igarashi, Masaru Tanokurab, and Masaharu Ishii
    Acta Cryst. F68, 911-913 (2012)
    PubMed
  17. Phylogenetic position of Aquificales based on the whole genome sequences of six Aquificales species
    Kenro Oshima, Yoko Chiba, Yasuo Igarashi, Hiroyuki Arai, and Masaharu Ishii
    Int. J. Evol. Biol., 2012:859264 (2012)
    PubMed

  18. Discovery and analysis of cofactor-dependent phosphoglycerate mutase homologs as novel phosphoserine phosphatases in Hydrogenobacter thermophilus
    Yoko Chiba, Kenro Oshima, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Biol. Chem. 287 (15), 11934-11941 (2012)
    PubMed
  19. A novel enzymatic system against oxidative stress in the thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus
    Yuya Sato, Masafumi Kameya, Shinya Fushinobu, Takayoshi Wakagi, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    PLoS ONE, 7 (4), e34825 (2012)
    PubMed

  20. Mechanism for folate-independent aldolase reaction catalyzed by serine hydroxymethyltransferase
    Yoko Chiba, Tohru Terada, Masafumi Kameya, Kentaro Shimizu, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    FEBS J. 279 (3), 504-514 (2012)
    PubMed
  21. Changes in the gene expression profile of Acetobacter aceti during growth on ethanol
    Kenta Sakurai, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Biosci. Bioeng., 113 (3), 343-348 (2012).
    PubMed

  22. Detecting weak protein-protein interactions by modified far-western blotting
    Yuya Sato, Masafumi Kameya, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Biosci. Bioeng., 112 (3) 304-307 (2011)
    PubMed

  23. Trade-off between oxygen and iron acquisition in bacterial cells at the air-liquid interface
    Kyosuke Yamamoto, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    FEMS Microbiol. Ecol. 77 (1), 83-94 (2011)
    PubMed

  24. Transcriptome response to different carbon sources in Acetobacter aceti
    Kenta Sakurai, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Microbiology, 157 (3), 899-910 (2011)
    PubMed

  25. Dissimilatory Fe(III) reduction by cytochrome c-552 in a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6
    Naoki Yoshida, Naoya Ohmura, Norio Matsumoto, Kengo Sasaki, Hiroshi Saiki, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Jpn. Soc. Extremophiles, 9 (2), 61-66 (2010)

  26. Complete genome sequence of the thermophilic, obligately chemolithoautotrophic hydrogen oxidizing bacterium Hydrogenobacter thermophilus TK-6
    Hiroyuki Arai, Haruna Kanbe, Masaharu Ishii, and Yasuo Igarashi
    J. Bacteriol., 192 (10), 2651-2652 (2010)
    PubMed

  27. Purification of three aminotransferases from Hydrogenobacter thermophilus TK-6 - novel types of alanine or glycine aminotransferase
    Masafumi Kameya, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    FEBS J., 277 (8), 1876-1885 (2010)
    PubMed

  28. Thiosulfate oxidation by a thermo-neutrophilic hydrogen-oxidizing Bacterium Hydrogenobacter thermophilus
    Ryoko Sano, Masafumi Kameya, Satoshi Wakai, Hiroyuki Arai, Yasuo Igarashi, Masaharu Ishii, and Yoshihiro Sambongi
    Biosci. Biotechnol. Biochem., 74 (4), 892-894 (2010)
    PubMed

  29. Differential expression of multiple terminal oxidases for aerobic respiration in Pseudomonas aeruginosa
    Takuro Kawakami, Miho Kuroki, Masaharu Ishii, Yasuo Igarashi, and Hiroyuki Arai
    Environ. Microbiol., 12 (6), 1399-1412 (2010)
    PubMed

  30. Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus
    Takeshi Ikeda, Masahiro Yamamoto, Hiroyuki Arai, Daijiro Ohmori, Masaharu Ishii, and Yasuo Igarashi
    FEBS J., 277 (2), 501-510 (2010)
    PubMed

  31. Carboxylation reaction catalyzed by 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus
    Masahiro Yamamoto, Takeshi Ikeda, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Extremophiles, 14 (1), 79-85 (2010)
    PubMed

  32. Siccanin rediscovered as a species-selective succinate dehydrogenase inhibitor
    Tatsushi Mogi, Takuro Kawakami, Hiroyuki Arai, Yasuo Igarashi, Kazunobu Matsushita, Mihoko Mori, Kazuro Shiomi, Satoshi Omura, Shigeharu Harada, and Kiyoshi Kita
    J. Biochem., 146(3), 383-387 (2009)

  33. Ferredoxin-NADP+ reductase from the thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6
    Takeshi Ikeda, Miyuki Nakamura, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    FEMS Microbiol. Lett., 297 (1), 124-130 (2009)

  34. A soluble NADH-dependent fumarate reductase in the reductive tricarboxylic acid cycle of Hydrogenobacter thermophilus TK-6.
    Akane Miura, Masafumi Kameya, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Bacteriol, 190 (21), 7170-7177 (2008)

  35. Coexistence of antibiotic-producing and antibiotic-sensitive bacteria mediated by resistant bacteria in biofilm
    Naoki Narisawa, Shin Haruta, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Appl. Environ. Microbiol., 74 (12), 3887-3894 (2008)

  36. Transcriptional activity of Pseudomonas aeruginosa fhp promoter is dependent on two regulators in addition to FhpR
    Taija Koskenkorva, Niina Aro-Kärkkäinen, Daniel Bachmann, Hiroyuki Arai, Alexander D. Frey, and Pauli T. Kallio
    Arch. Microbiol. 189 (4), 385-396 (2008)

  37. Transcriptome dynamics during the transition from anaerobic photosynthesis to aerobic respiration in Rhodobacter sphaeroides 2.4.1
    Hiroyuki Arai, Jung Hyeob Roh, and Samuel Kaplan
    J. Bacteriol., 190 (1), 286-299 (2008)

  38. Sequencing and reverse transcription-polymerase chain reaction (RT-PCR) analysis of four hydrogenase gene clusters from an obligately autotrophic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus TK-6
    Yasufumi Ueda, Masahiro Yamamoto, Takashi Urasaki, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Biosci. Bioeng., 104 (6), 470-475 (2007)

  39. The anaerobic regulatory network required for Pseudomonas aeruginosa nitrate respiration
    Kerstin Schreiber, Robert Krieger, Beatrice Benkert, Martin Eschbach, Hiroyuki Arai, Max Schobert, and Dieter Jahn
    J. Bacteriol. 189(11), 4310-4313 (2007)

  40. A novel ferredoxin-dependent glutamate synthase from the hydrogen-oxidizing chemoautotrophic bacterium, Hydrogenobacter thermophilus TK-6
    Masafumi Kameya, Takeshi Ikeda, Miyuki Nakamura, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Bacteriol. 189(7), 2805-2812 (2007)

  41. Purification and properties of glutamine synthetase from Hydrogenobacter thermophilus TK-6
    Masafumi Kameya, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Biosci. Bioeng., 102 (4), 311-315 (2006)

  42. Role of two 2-oxoglutarate:ferredoxin oxidoreductases in Hydrogenobacter thermophilus under aerobic and anaerobic conditions
    Masahiro Yamamoto, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    FEMS Microbiol. Lett., 263 (2), 189-193 (2006)

  43. Gene structure and expression profile of cytochrome bc nitric oxide reductase from Hydrogenobacter thermophilus TK-6
    Miho Suzuki, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Biosci. Biotechnol. Biochem. 70 (7), 1666-1671 (2006)

  44. Purification, characterization, and gene cloning of thermophilic cytochrome cd1 nitrite reductase from Hydrogenobacter thermophilus TK-6
    Miho Suzuki, Tadao Hirai, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Biosci. Bioeng., 101 (5), 391-397 (2006)

  45. Anabolic five subunit-type pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6
    Takeshi Ikeda, Toshiro Ochiai, Susumu Morita, Ayako Nishiyama, Eio Yamada, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 340 (1), 76-82 (2006)

  46. The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus MH-110
    Koichi Toyoda, Yoichi Yoshizawa, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Microbiology, 151 (11), 3615-3625 (2005)

  47. Two tandemly arranged ferredoxin genes in the Hydrogenobacter thermophilus genome: Comparatives characterization of the recombinant [4Fe-4S] ferredoxins
    Takeshi Ikeda, Masahiro Yamamoto, Hiroyuki Arai, Daijiro Ohmori, Masaharu Ishii, and Yasuo Igarashi
    Biosci. Biotechnol. Biochem., 69(6), 1172-1177 (2005)

  48. Transcriptional regulation of the flavohemoglobin gene for aerobic nitric oxide detoxification by the second nitric oxide-responsive regulator of Pseudomonas aeruginosa
    Hiroyuki Arai, Michiko Hayashi, Azusa Kuroi, Masaharu Ishii, and Yasuo Igarashi
    J. Bacteriol., 187 (12), 3960-3967 (2005)

  49. CO2-responsive expression and gene organization of three ribulose-1,5-bisphosphate carboxylase/oxygenase enzymes and carboxysomes in Hydrogenovibrio marinus strain MH-110
    Yoichi Yoshizawa, Koichi Toyoda, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Bacteriol., 186 (17), 5685-5691 (2004)

  50. Occurrence, biochemistry and possible biotechnological application of the 3-hydroxypropionate cycle
    Masaharu Ishii, Songkran Chuakrut, Hiroyuki Arai, and Yasuo Igarashi
    Appl. Microbiol. Biotechnol., 64, 605-610 (2004)

  51. Characterization of two different 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6
    Masahiro Yamamoto, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 312 (4), 1297-1302 (2003)

  52. Characterization of a bifunctional archaeal acyl coenzyme A carboxylase
    Songkran Chuakrut, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    J. Bacteriol., 185 (3), 938-947 (2003)

  53. Need for cytochrome bc1 complex for dissimilatory nitrite reduction of Pseudomonas aeruginosa
    Norio Hasegawa, Hiroyuki Arai, and Yasuo Igarashi
    Biosci. Biotechnol. Biochem., 67 (1), 121-126 (2003)

  54. Transcriptional regulation of the nos genes for nitrous oxide reductase in Pseudomonas aeruginosa
    Hiroyuki Arai, Masayuki Mizutani, and Yasuo Igarashi
    Microbiology, 149 (1), 29-36 (2003)

  55. A novel five-subunit-type 2-oxoglutalate: ferredoxin oxidoreductases from Hydrogenobacter thermophilus TK-6
    Na-Rae Yun, Masahiro Yamamoto, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 292 (1), 280-286 (2002)

  56. Meta-cleavage enzyme gene tesB is necessary for testosterone degradation in Comamonas testosteroni TA441
    Masae Horinouchi, Takako Yamamoto, Katsuhiko Taguchi, Hiroyuki Arai, and Toshiaki Kudo
    Microbiology, 147 (12), 3367-3375 (2001)

  57. Two c-type cytochromes, NirM and NirC, encoded in the nir gene cluster of Pseudomonas aeruginosa act as electron donors for nitrite reductase
    Norio Hasegawa, Hiroyuki Arai, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 288 (5), 1223-1230 (2001)

  58. The genes for anabolic 2-oxoglutarate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus TK-6
    Na-Rae Yun, Hiroyuki Arai, Masaharu Ishii, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 282 (2), 589-594 (2001)

  59. Arrangement and regulation of the genes for meta-pathway enzymes required for degradation of phenol in Comamonas testosteroni TA441
    Hiroyuki Arai, Tohru Ohishi, Mee Young Chang, and Toshiaki Kudo
    Microbiology, 146 (7), 1707-1715 (2000)

  60. Stabilization of Pseudomonas aeruginosa cytochrome c551 by systematic amino acid substitutions based on the structure of thermophilic Hydrogenobacter thermophilus cytochrome c552
    Jun Hasegawa, Hideto Shimahara, Masayuki Mizutani, Susumu Uchiyama, Hiroyuki Arai, Masaharu Ishii, Yuji Kobayashi, Stuart J. Ferguson, Yoshihiro Sambongi, and Yasuo Igarashi
    J. Biol. Chem., 274 (53), 37533-37537 (1999)

  61. The cbbQ genes located downstream of the form I and form II RubisCO genes, affect the activity of both RubisCOs
    Nobuhiro R. Hayashi, Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 265 (1), 177-183 (1999)

  62. Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441
    Hiroyuki Arai, Takako Yamamoto, Tohru Ohishi, Takeshi Shimizu, Tadashi Nakata, and Toshiaki Kudo
    Microbiology, 145 (10), 2813-1820 (1999)

  63. Adaptation of Comamonas testosteroni TA441 to utilization of phenol by spontaneous mutation of the gene for a trans-acting factor
    Hiroyuki Arai, Saiko Akahira, Tohru Ohishi, and Toshiaki Kudo
    Mol. Microbiol., 33 (6), 1132-1140 (1999)

  64. Effect of nitrogen oxides on expression of the nir and nor genes for denitrification in Pseudomonas aeruginosa
    Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    FEMS Microbiol Lett., 170 (1), 19-24 (1999)

  65. Two sets of biphenyl and PCB degradation genes on a linear plasmid in Rhodococcus erythropolis TA421
    Hiroyuki Arai, Saori Kosono, Katsuhiko Taguchi, Michihisa Maeda, Eun Song, Fumie Fuji, Seon-Yong Chung, and Toshiaki Kudo
    J. Ferment. Bioeng., 86 (6), 595-599 (1998)

  66. Adaptation of Comamonas testosteroni TA441 to utilize phenol: organization and regulation of the genes involved in phenol degradation
    Hiroyuki Arai, Saiko Akahira, Tohru Ohishi, Michihisa Maeda, and Toshiaki Kudo
    Microbiology, 144 (10), 2895-2903 (1998)

  67. The role of the nirQOP operon in energy conservation during anaerobic growth of Pseudomonas aeruginosa
    Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    Biosci. Biotechnol. Biochem., 62 (10), 1995-1999 (1998)

  68. Activation of a consensus FNR-dependent promoter by DNR of Pseudomonas aeruginosa in response to nitrite
    Norio Hasegawa, Hiroyuki Arai, and Yasuo Igarashi
    FEMS Microbiol. Lett., 166 (2), 213-217 (1998)

  69. The nirQ gene, which is required for denitrification of Pseudomonas aeruginosa, can activate the RubisCO from Pseudomonas hydrogenothermophila
    Nobuhiro R. Hayashi, Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    Biochem. Biophys. Acta, 1381 (3), 347-350 (1998)

  70. Regulation of Pseudomonas aeruginosahemF and hemN by the dual action of the redox response regulators Anr and Dnr
    Alexandra Rompf, Christoph Hungerer, Tamara Hoffmann, Maja Lindenmeyer, Ute R?mling, Ulrich Gro?, Manfred O. Doss, Hiroyuki Arai, Yasuo Igarashi, and Dieter Jahn
    Mol. Microbiol., 29 (4), 985-997 (1998)

  71. Heterologous expression of Hydrogenobacter thermophilus cytochrome c-552 in the periplasm of Pseudomonas aeruginosa
    Yan Zhang, Hiroyuki Arai, Yoshihiro Sambongi, Yasuo Igarashi, and Tohru Kodama
    J. Ferment. Bioeng., 85 (3), 346-349 (1998)

  72. Three of the seven bphC genes of Rhodococcus erythropolis TA421, isolated from a termite ecosystem, are located on an indigenous plasmid associated with biphenyl degradation
    Saori Kosono, Michihisa Maeda, Fumie Fuji, Hiroyuki Arai, and Toshiaki Kudo
    Appl. Environment. Microbiol., 63 (8), 3282-3285 (1997)

  73. The novel genes, cbbQ and cbbO, located downstream from the RubisCO genes of Pseudomonas hydrogenothermophila, affect the conformational states and activity of RubisCO
    Nobuhiro R. Hayashi, Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    Biochem. Biophys. Res. Commun., 241 (2), 565-569 (1997)

  74. Cascade regulation of the two CRP/FNR-related transcriptional regulators (ANR and DNR) and the denitrification enzymes in Pseudomonas aeruginosa
    Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    Mol. Microbiol., 25 (6), 1141-1148 (1997)

  75. Gene cluster for dissimilatory nitrite reductase (nir) from P. aeruginosa: Sequencing and identification of a locus for heme d1 biosynthesis
    Shinji Kawasaki, Hiroyuki Arai, Tohru Kodama, and Yasuo Igarashi
    J. Bacteriol., 179 (1), 235-242 (1997)

  76. Sequencing and characterization of the downstream region of the genes for nitrite reductase and cytochrome c-551 (nirSM) from P. aeruginosa: Identification of the gene essential for the biosynthesis of heme d1
    Shinji Kawasaki, Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    Gene, 167 (1-2), 87-91 (1995)

  77. Expression of the nir and nor genes for denitrification of P. aeruginosa requires a novel CRP/FNR related transcriptional regulator, DNR, in addition to ANR
    Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    FEBS Lett., 371 (1), 73-76 (1995)

  78. Production of recombinant cytochrome c-551 in a Pseudomonas aeruginosa mutant strain
    Hiroyuki Arai, Yan Zhang, Yoshihiro Sambongi, Yasuo Igarashi, and Tohru Kodama
    J. Ferment. Bioeng., 79 (5), 489-492 (1995)

  79. The structural genes for nitric oxide reductase from Pseudomonas aeruginosa
    Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    Biochim. Biophys. Acta, 1261 (2), 279-284 (1995)

  80. Genes encoding RubisCO in Pseudomonas hydrogenothermophila are followed by a novel cbbQ gene similar to nirQ of the denitrification gene cluster from Pseudomonas species
    Keiichi Yokoyama, Nobuhiro R. Hayashi, Hiroyuki Arai, Seon Yong Chung, Yasuo Igarashi, and Tohru Kodama
    Gene, 153 (1), 75-79 (1995)

  81. Structure and ANR-dependent transcription of the nir genes for denitrification from Pseudomonas aeruginosa
    Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    Biosci. Biotechnol. Biochem., 58 (7), 1286-1291 (1994)

  82. Construction of novel expression vectors effective in Pseudomonas cells
    Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    Agric. Biol. Chem., 55 (9), 2431-2432 (1991)

  83. Nitrite activate the transcription of the Pseudomonas aeruginosa nitrite reductase and cytochrome c-551 operon under anaerobic conditions
    Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    FEBS Lett., 288 (1-2), 227-228 (1991)

  84. Anaerobically induced expression of the nitrite reductase cytochrome c-551 operon from Pseudomonas aeruginosa
    Hiroyuki Arai, Yasuo Igarashi, and Tohru Kodama
    FEBS Lett., 280 (2), 351-353 (1991)

  85. Cloning and sequencing of the gene encoding cytochrome c-551 from Pseudomonas aeruginosa
    Hiroyuki Arai, Yoshihiro Sanbongi, Yasuo Igarashi, and Tohru Kodama
    FEBS Lett., 261 (1), 196-198 (1990)

総説等：
  1. 古くて新しい酢酸菌の酸化発酵
    桜井健太、石井正治、新井博之
    バイオサイエンスとインダストリー、75 (1), 17-21 (2017)

  2. “電子の流れ”で代謝を読み解く：Hydrogenobacter thermophilusの新規peroxidaseの発見
    佐藤由也、新井博之、石井正治
    バイオサイエンスとインダストリー、71 (4), 308-313 (2013)

  3. Regulation and function of versatile aerobic and anaerobic respiratory metabolism in Pseudomonas aeruginosa
    Hiroyuki Arai
    Front. Microbio. 2:103 (2011)
    PubMed
  4. ゲノム解析から見えてきた好熱性絶対独立栄養細菌Hydrogenobacter thermophilusの特徴
    新井博之
    バイオサイエンスとインダストリー, 68 (5), 322-326 (2010)

  5. 微生物由来脱窒遺伝子群の発現調節に関する研究
    新井博之
    日本農芸化学会誌, 75 (8), 863-869 (2001)

  6. アジア地域の微生物研究ネットワークと環境保全
    工藤俊章、新井博之
    農業および園芸, 74 (11), 1101-1198 (1999)

  7. 芳香族分解性シュードモナス属細菌の多様性と進化
    新井博之、工藤俊章
    日本農芸化学会誌, 71 (9), 898-901 (1997)

  8. 進化的観点から見た環境汚染物質分解微生物について
    新井博之、工藤俊章
    バイオサイエンスとインダストリー, 55 (2), 87-91 (1997)

  9. Arrangement and transcriptional regulation of the denitrification genes in Pseudomonas aeruginosa
    Hiroyuki Arai, Shinji Kawasaki, Yasuo Igarashi, and Tohru Kodama
    In Molecular Biology of Pseudomonads., Nakazawa, T., Furukawa, K., Haas, D., and Silver, S. (eds). Washington D.C.: ASM Press, pp. 298-308 (1996)

  10. Pseudomonas属細菌の脱窒遺伝子クラスター
    新井博之
    生物工学会誌 vol.72 (2) 123 (1994)
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